FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20562948
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG215160.1046348023639412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC71950.049.970799
TTCCGAC76250.047.2108088
CGGGGAT158000.036.820628
GTTTCCG84750.036.311596
TCGGGGA161750.035.790467
TTTCCGA101750.035.752157
CCCCGTT55900.034.0650252
CCGTTTC106750.032.3897254
TGTCGGG37450.031.9595725
ATAGCGC135000.031.6715194-95
GCCCCGT40850.031.6198161
TCCGTTT106850.031.5114153
GTTCCGT28700.031.4379481
CCCCGTA31700.031.0089632
CCGATCG142850.031.00825786-87
CTCCGTT81550.030.51292
ATCGGGG116300.030.462756
CGTTTCC103400.030.454065
CCCCCGT43350.030.453571
GTTCACC264200.030.2883037
Produced by FastQC (version 0.11.5)