FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21141882
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC212070.10030800474621891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT28350.047.5761075
TGCGACA29650.045.490144
CTGCGAC31550.043.204483
GCTGCGA35450.038.5853542
CGACATC37650.036.2026676
CTAGGCG75850.034.5000467
TAGGCGG77650.033.8227048
GGCTGCG43550.031.660781
GACCTCG40600.027.7233684
ACCTCGT42550.026.5644635
CTCGTGA44950.025.3574287
CGTGATC45150.024.824419
GCTAGGC109650.024.7748496
CGGTCGA52650.024.31564168-69
CCTCGTG49850.022.9601946
TCGTGAT51850.022.2577978
CGCGCTC60700.022.06787962-63
TCGGTCG57900.021.86472368-69
CCGATAG41200.021.84629490-91
GGCTAGG129400.021.8009685
Produced by FastQC (version 0.11.5)