FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21141882
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC39750.042.0952349
TTCCGAC43750.038.5487448
GGGCGAC37100.036.574141
GTTCCGT28550.033.9479751
GCCCCGT29150.032.6446881
CGGTTCA66950.032.2423255
GGTCCGT22100.031.6958331
GAGCGAG48150.029.9960449
GTCCCGT16950.029.8899671
ATAGCGC77800.029.66244594-95
GGCGACA46350.029.3710482
TTTCCGA58400.029.330967
CCCCGTT38800.029.1818032
GTTTCCG50750.029.1536376
CCGATCG81100.028.86529586-87
GACCCGT10400.028.8053341
TGGTCGT66650.028.7360614-15
CCGGTTC55950.028.3454444
CGATCGG81250.028.21626986-87
CGGGGAT68350.028.0890798
Produced by FastQC (version 0.11.5)