FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18475354
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC389870.21102166702732733No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC263480.14261161112257983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA57100.064.2641454
GCGACAT57600.063.7080235
CTGCGAC63150.058.257553
CGACATC66850.054.819786
GCTGCGA70000.052.5566332
GGCTGCG79350.041.970961
ACTCGGG129850.032.0265731
CTCGGGA153350.029.895552
CTAGGCG179800.029.5580047
TAGGCGG184550.028.7201658
TCGGGAG162300.028.1593343
TTGATCG68950.026.88389822-23
GCTAGGC205850.025.9556676
TGATCGC72350.025.35828424-25
TGGCTAG222050.024.8522724
GGCTAGG220350.024.7864915
GACATCT157850.023.907147
GATCGCC77750.023.68852624-25
TCGCCAG79000.023.31370726-27
ATTCGGC81650.022.5001138-39
Produced by FastQC (version 0.11.5)