FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18475354
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG206550.11179758720726002No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG202290.10949181271438697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC84800.058.4733059
TTCCGAC88750.055.919058
TTTCCGA110650.045.4521067
GTTTCCG96950.044.1354566
CCGTTTC105600.040.030054
CGTTTCC105250.039.2608345
CGGGGAT141650.038.9248928
GTTCCGT29050.038.089591
TGTCGGG33350.038.0257535
TCCGTTT111250.037.6937183
CTCCGTT85250.037.6564562
TCGGGGA148300.037.4674537
GCTCCGT72800.036.7912251
GTCCCGT29250.035.8808671
CCCCGTT50100.035.071242
CCCGTTA23050.032.965323
GCCCCGT35450.032.4187051
ATAGCGC134050.032.2648194-95
CTGTCGG39200.032.108494
CCGATCG142550.031.22300786-87
Produced by FastQC (version 0.11.5)