FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18972909
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC77950.051.293859
TTCCGAC81650.048.915788
TTTCCGA98900.040.784687
GTTTCCG89450.038.742916
GTTCCGT24650.037.353991
CCGTTTC97100.036.8869864
CGTTTCC94800.036.0624545
CGGTTCA113550.035.88865
GTCCCGT25950.035.6524661
TCCGTTT99650.035.279913
CTCCGTT76800.034.7641532
CCCCGTT50000.034.541112
GCTCCGT65650.034.49341
GTCCGGT18900.032.8683242
GCCCCGT35450.032.187791
CCCGTTA23850.031.9565323
CCCCGTA24400.031.2375162
CCCCCGT36450.031.1838551
CCGGTTC91300.030.8824024
ATAGCGC147400.030.71421494-95
Produced by FastQC (version 0.11.5)