FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677867
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451620.6762937926137194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243970.3653412085026551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203220.30431872931880793No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116860.17499599797360443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76350.076.106511
GTATCAA193450.058.998711
CAACGCA238000.049.92815
ATCAACG238900.049.790173
TCAACGC241400.049.3484424
AACGCAG250650.047.5212366
GCAGCGC7900.043.656228
ACGCAGA272700.043.654017
CGCAGAG273400.043.520498
TATCAAC281100.042.3154452
TCTGTCG11300.041.5713238
GCAGAGT302300.039.3795859
CGCTCTG11700.032.5327385
GTCTCGC11000.032.4410441
TGCAGCG10800.031.9337147
CTCGCTC11700.031.0079983
GTACTTT446200.030.2707961
AGAGTAC320000.028.83015610-11
CAGAGTA325550.027.64457710-11
TACTTTT527850.027.3907662
Produced by FastQC (version 0.11.5)