FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677867
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA38350.041.911525
GCAGCGC7100.041.057528
CGCAGAG39200.040.6727688
ACGCAGA40700.039.172897
TCTGTCG10450.035.2964028
TCAACGC45800.035.2240144
AACGCAG46200.034.7664646
ATCAACG46750.034.635573
GTCTCGC9950.032.3168871
GTATCAA60850.029.2596721
TGCAGCG10050.029.005167
CTCGCTC10000.027.9790443
GCAGAGT60100.026.8262259
TCTCGCT11150.026.6950152
ACGGGAC4200.025.5128063
CGCTCTG10850.025.2384785
GGTATCA31050.023.9721641
GTACGGG55050.023.7971061
GAGTACG43850.023.4928281
CTGTCGC17400.021.5405149
Produced by FastQC (version 0.11.5)