FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24405506
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC49950.049.159049
TTCCGAC53850.045.507318
GTCCCGT28900.036.4803471
CGGTGTG52100.036.101515
GTTCCGT30950.035.905341
GTTTCCG60700.035.0516326
GCTCCGT56300.034.246911
CTCCGTT69450.034.1208342
GGTGTGC159400.033.399216
TTTCCGA74700.033.1869287
CCGGTGT38700.033.1369634
TCCGTTT96000.032.700043
CCCGTTA28650.032.4899833
CCCCGAA48850.032.1776852
TCCGATC96050.032.14166316-17
CCGATCT100100.032.098616-17
GTGCTCT203300.031.9359239
CCGTTTC87950.030.8901544
CCCCGTT57600.030.1751842
CCCCGTA32250.029.597682
Produced by FastQC (version 0.11.5)