FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20653187
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC410400.1987102523208646No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC385780.18678957392871134No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT279480.1353205197822496No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT277550.1343860393071539No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC216200.10468118068170303No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG211540.10242487031178286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA75750.068.722854
GCGACAT80750.064.647155
CTGCGAC83500.062.6282843
GCTGCGA95900.055.074182
ACTCGGG172850.041.506051
GGCTGCG117350.040.9375951
ACCCGAC91900.040.4465983
CCGACTC99100.037.3664635
CCCGACT100400.037.352894
CTCGGGA207850.036.90712
CGACTCC107250.034.215766
TCGGGAG226100.034.0748753
AACCCGA110600.033.436512
CGACATC157700.033.1308366
CTAGGCG203100.030.0668857
TAGGCGG206600.029.4886828
CCCTTAG104800.028.546283
GACTCCC132450.028.2785847
TAGAGCC107700.027.6456267
ACATCTG191900.027.4981028
Produced by FastQC (version 0.11.5)