FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20653187
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA330980.16025613867728986No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG238680.11556569937608177No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG223540.10823511160771458No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG215830.10450203157507847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC113600.052.8437968
TCCGACC124250.048.2796179
TTTCCGA139500.043.679517
GTTTCCG125500.041.362126
CGTTTCC126350.041.1633265
CCGTTTC130000.040.372274
GTAGCAA292450.039.4974259
GTTCCGT35250.038.2600561
TCACCCC370900.036.8033039
TCCGTTT132600.036.5682643
GCCCCGT49450.036.3964731
CGGGGAT172800.035.976698
AGTAGCA322800.035.6488238
TAGTAGC323300.035.167687
GCTCCGT97800.035.154921
ATAGCGC214100.034.6500994-95
TCGGGGA183450.033.9657677
CTCCGTT113400.033.6263242
TGTCGGG38750.033.217675
TCAGTCG228850.032.75583684-85
Produced by FastQC (version 0.11.5)