FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20935679
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT389770.18617499819327568No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT324300.1549030246403759No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC246280.11763649987182169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA59150.060.8349384
GCGACAT65750.054.874885
CTGCGAC69850.052.1267743
GCTGCGA76850.047.3170362
ACTCGGG235400.040.6840931
CTCGGGA283050.037.200732
TCGGGAG300100.035.0871933
GGCTGCG99100.033.8678361
CGACATC106350.033.8357246
CGGGAGG428500.025.5248264
TTGATCG78050.023.65863622-23
TGATCGC78600.023.37231324-25
GCGCGGT46750.022.3106546
TCGCCAG84500.021.74040226-27
GGAGGCT624450.021.1749326
TTCGGCT87250.021.10996240-41
ACATCTG175850.020.8674728
GACATCT176450.020.8233817
ACCCGAC45150.020.6859043
ATTCGGC88450.020.5824438-39
Produced by FastQC (version 0.11.5)