FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20935679
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG478070.22835180077034997No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA366940.17527016916910124No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA227330.10858496636292524No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG225190.10756278790862239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC143500.061.821498
TCCGACC143750.061.3558359
TTTCCGA165300.053.6683857
GTTTCCG152200.050.955036
CGTTTCC149700.050.0666435
CCGTTTC158350.047.9306874
GCTCCGT116100.046.711581
TCCGTTT156750.046.0816353
CTCCGTT129550.045.3065832
TCACCCC508800.041.2131169
GTTCCGT40850.041.153031
CGGGGAT138550.039.3504648
ATAGCGC289300.039.07016494-95
GTCGGTT43650.039.0585753
TCGGGGA151400.036.41847
CGATCGG316300.036.12405486-87
GTTCACC581900.035.8776367
CCGATCG315800.035.79769586-87
TGTCGGG40650.035.637125
CGGCATA321750.035.49739590-91
Produced by FastQC (version 0.11.5)