FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21282471
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1954670.9184412843790555No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC1748860.8217372879305228No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT1515720.7121917375101792No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT1137160.5343176551256666No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC1015900.4773411884362488No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT782590.36771576007316065No Hit
NGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC461510.21684981974132606No Hit
NGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC441650.2075181965477599No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA415260.19511832061230108No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA376630.1769672327992365No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT367850.17284177199160755No Hit
NCTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT354520.16657840153993395No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG333860.15687088214521708No Hit
GAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTG313930.14750636803405018No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC301440.14163768859358483No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG294630.13843787218128947No Hit
NCTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT273030.1282886747502205No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC254860.11975113228158515No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT244170.1147282192937089No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC239050.11232248360634439No Hit
NGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC234560.11021276617738608No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA231240.10865279694261065No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT230440.10827690074145994No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA220480.10359699303713371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA286400.083.9948654
GCGACAT288650.083.459035
CTGCGAC302450.079.615923
GGCTGCG278650.078.142781
GCTGCGA308900.078.0302352
ACATCTG379400.063.5314258
CGACATC399850.060.1991656
CATCTGT401300.060.0761649
GACATCT414750.058.253697
ACTCGGG630700.052.551331
TACGGGT29950.050.1808173
CTCGGGA751050.048.0765652
TCGGGAG779700.046.322153
ACGGGTG94950.043.490914
CGGGAGG987100.037.3434564
CCCGACT142500.035.366634
ACCCGAC141900.035.3491173
TGATCGC345250.034.98166724-25
TTGATCG346850.034.80691522-23
CCGACTC144400.034.8044175
Produced by FastQC (version 0.11.5)