FastQCFastQC Report
Fri 6 Jan 2023
EGAF00004913671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21282471
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG1319520.6200031941779692No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1139910.5356097983171221No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA841390.3953441308577373No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG724780.340552560837508No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA605090.2843137904428485No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG561510.26383684488516396No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA526070.2471846431741878No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT487320.22897717093095066No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA466980.219420010016694No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA450170.2115214910900149No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA380100.1785976825717277No Hit
TCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGA373200.1753555778368029No Hit
CCTCTCTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG343400.1613534443439392No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG317250.1490663372688256No Hit
CCTCTTTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG315960.1484602046444701No Hit
GGGGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA315490.14823936562629406No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG312460.14681565876443575No Hit
TGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG299290.14062746755299232No Hit
GGGTCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA297680.13987097644817653No Hit
GGGCCCGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA290500.13649730804284896No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG288230.13543070257208387No Hit
GGGTCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA271760.12769193953089375No Hit
CCTCCATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG259730.12203940040609006No Hit
GGTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA255500.12005184924250571No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG240070.11280175126281153No Hit
ATTGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACG231660.10885014244821478No Hit
GGGCAGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA221360.10401047885839948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)