FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19219121
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCNGGTTCACCCCTCCTTAGGCAACCTGGTGG378660.19702253812752413No Hit
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG300850.15653681560150434No Hit
GGGCCGGTTCACCCCTCCTTNGGCAACCTGGTGGTCCCCCGCTCCCGGGA276250.14373706268876707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC148200.051.249969
TTCCGAC150450.050.5421758
TTTCCGA172700.044.1579937
GTTTCCG158250.041.623796
CGGTTCA235850.040.0643355
GTTCCGT35450.040.0140881
CGTTTCC157900.039.9788745
TCACCCC505600.039.919979
CCGTTTC159400.038.5524864
TCCGTTT151450.037.0576553
TGTCGGG40300.037.056225
GTTCACC556800.036.2808957
CGGGGAT199050.036.144628
TCGGGGA204000.035.4186677
TTCACCC571150.035.400188
CTCCGTT128950.035.0184862
CCGGTTC188700.034.760764
TGGTCGT206350.034.6286114-15
GCTCCGT111700.034.626981
GACGGTT20700.034.263633
Produced by FastQC (version 0.11.5)