FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15551871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG315880.20311382469671976No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG210680.13546923067970407No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG165660.1065209452933348No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA157230.10110037563968992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC90650.055.901429
TTCCGAC94800.053.654658
TTTCCGA115600.044.2458887
TGTCGGG39950.041.962275
CGGGGAT202250.041.4459768
GTTTCCG107850.041.0394026
TCGGGGA209950.039.9927647
CGTTTCC108250.039.48355
CCGTTTC108900.038.940824
GTTCCGT28300.037.4087141
TCACCCC308900.036.4535529
CTGTCGG44900.035.9595384
GTTCACC315750.035.5715527
CCCCGTT45500.035.3710752
TCCGTTT112750.035.2920463
TTCACCC321700.035.0031178
GCTCCGT81800.034.8799251
CTCCGTT89550.034.0352862
GTCGGGG51450.033.9678236
ATAGCGC178150.033.790394-95
Produced by FastQC (version 0.11.5)