FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21495899
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG244800.11388218748143542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC81700.051.679589
TTCCGAC109600.038.61058
CGGGGAT188050.036.0150878
TTTCCGA118650.035.905087
GTTTCCG104950.035.8392376
TGTCGGG42350.035.4359975
TCGGGGA198300.034.3445367
CCCCGTT63550.032.273032
CGTTTCC115400.032.181925
GTCCCGT34300.031.4198591
ATAGCGC144450.031.0916794-95
CCGATCG151350.030.76395886-87
GTTCCGT39550.030.6102031
CCGTTTC130900.030.5830444
GCTCCGT81300.030.1322561
CGATCGG153150.029.75090486-87
GCCCCGT47300.029.5092851
TCACCCC282950.029.3743069
GATCGGC160800.029.2612788-89
CCCCGTA36250.029.205752
Produced by FastQC (version 0.11.5)