FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23330128
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG477620.20472240872403272No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA307640.1318638286082271No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG254780.10920643041478384No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA254230.10897068374421262No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG242000.10372853505132933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC112650.049.6668478
TCCGACC122500.045.5958829
TGTCGGG62100.044.8141065
CGGGGAT307400.042.1959088
TCGGGGA321800.040.3208277
GTAGCAA325850.039.4862259
CGTTTCC127200.038.7915535
TTTCCGA145700.038.692437
TCTGTCG71950.037.551223
GTTTCCG128250.037.2526136
CCGTTTC135400.037.0367244
CTGTCGG74100.036.6576924
AGTAGCA355450.036.077618
TCACCCC366950.035.9696359
GCCCCGT56750.035.8902321
TAGTAGC352800.035.875957
GTTCCGT40500.034.3476871
GTCGGGG84250.034.216456
TCCGTTT138100.034.0395773
ATAGCGC218250.033.10845694-95
Produced by FastQC (version 0.11.5)