FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22647694
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG416610.18395250306719968No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG235570.10401500479474864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC107450.050.5670939
TTCCGAC109750.049.63728
TGTCGGG53950.046.655525
CGGGGAT281750.040.9797638
TCGGGGA289050.040.0911877
CTGTCGG62250.039.670034
TTTCCGA137900.039.5719877
GTTTCCG125800.037.4879846
GTCGGGG73750.036.2551576
CGTTTCC129300.036.0687945
CCGTTTC137300.035.902554
TCTGTCG75550.033.123653
TCACCCC365550.033.0535939
GCTCCGT99300.032.6072851
GTTCCGT41850.032.558621
ATAGCGC200700.032.4710494-95
CTCCGTT112950.032.4078752
CCCCGTT64700.032.360612
TCCGTTT147700.032.214343
ATCGGGG213500.031.8765816
Produced by FastQC (version 0.11.5)