FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748749
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG194420.22222605769121961No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA127120.14530077385921117No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC116980.13371054535911364No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC112080.12810974460462862No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG99210.11339906996988942No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG97280.1111930402849596No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG93400.10675812050385718No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG93130.10644950495207943No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC90190.10308902449938843No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG89120.10186599249789885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.048.2630581
CAACGCA76900.047.128465
GCAGCGC13100.046.7731638
CGCAGAG80200.045.172468
ATCAACG79850.045.0149573
ACGCAGA81250.044.8073127
TCAACGC80700.044.540564
GTATCAA87500.043.7583771
GCTCTAG16450.040.1805041
AACGCAG92800.039.293976
GCAGAGT94800.038.157359
TGCAGCG16400.037.723367
TGGTCCT38900.030.1376175
CTAGCCT73950.026.1539634
TAGCGTG9000.025.7813159
GAGTACG92250.025.22132712-13
CGATACC9150.025.020607118-119
AGTACGG113750.024.6653812-13
AATGCGT45700.024.6013556
GCCTTTC58650.024.5452927
Produced by FastQC (version 0.11.5)