FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354095
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG186100.22276500327085097No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA119040.14249299295734608No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC111640.13363506160751104No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC109010.13048690492506967No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG96210.11516507772535506No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG93750.1122204140604099No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG91350.1093475714604634No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG87950.1052777111105392No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG87500.10473905312304924No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC86660.10373355821306797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA71750.050.2735065
ATCAACG75100.048.347983
ACGCAGA75750.047.9061167
GGTATCA26850.047.675991
CGCAGAG75750.047.6716588
TCAACGC76300.047.4315644
GTATCAA83450.045.876491
GCTCTAG14300.044.9669381
AACGCAG85250.042.566596
GCAGCGC13100.041.7802248
GCAGAGT88250.040.6501359
TGCAGCG15950.034.6869477
TGGTCCT40150.033.8016365
AATGCGT42150.025.827776
AAATGCG42700.025.7888685
GAGTACG87400.025.77005612-13
CTAGCCT72150.025.739894
TATACGG6300.025.510072
ACGGGAC11000.025.4327663
GCCTTTC57200.025.1688357
Produced by FastQC (version 0.11.5)