FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17796750
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC521070.29278941379746304No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT519300.29179485018332No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC496700.2790959023417197No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT473540.26608229030581426No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG402160.2259738435388484No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT375150.21079691516709514No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT230940.12976526613005182No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT208840.11734726846193827No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC205220.11531318920589434No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC186610.10485622374871817No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG184480.10365937600966468No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG181260.10185005689241013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA78150.067.2257164
GCGACAT86950.060.637595
CTGCGAC95300.055.7304233
GCTGCGA103900.050.9859352
GGCTGCG135850.039.6970061
ACTCGGG274050.036.6345831
CTCGGGA291300.034.593242
TCGGGAG305650.033.152013
TATCGCT16100.033.053211
TCGACCG34300.032.8244633
CGACATC172850.030.5301936
ACATCTG179750.029.5414738
CCTCGAC40350.028.9676421
GCTCGCG40500.028.4977479
CATCTGT192600.027.4225469
ACCGACT149850.027.0047197
CGACTGA153350.026.6982469
GACATCT203950.026.1757667
CTAGGCG214400.026.0297437
CTCGACC44500.025.730492
Produced by FastQC (version 0.11.5)