FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17796750
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG616700.3465239439785354No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA593670.33358337898773655No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG566290.31819854748760307No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA336780.1892367988537233No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG296130.16639554974925197No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA293660.16500765589222752No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG281350.15809066262098417No Hit
GGGCTGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA220370.12382597946254233No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG211600.11889811341958503No Hit
GGGTCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA211370.11876887633978114No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA206510.11603804065349009No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG200580.11270597159593745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)