FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13351453
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC707140.5296352389511464No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC362910.2718131127750665No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG357630.26785848701261206No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG271000.2029741631865835No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC197470.14790150555149317No Hit
AACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGA142220.10652024165459745No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATT138010.10336702679476159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA53800.067.5426254
GCGACAT56500.064.480185
CTGCGAC64600.056.622783
CGACATC64750.056.337916
GCTGCGA67000.054.4596332
CAGACGT56950.046.8729066
TCAGACG56950.046.3724825
GGCTGCG87600.042.5787471
AGACGTG64750.041.2248957
CTAGGCG200650.039.0341347
TCGACCG9750.038.4906653
TAGGCGG203500.038.4886178
TGGCTAG212550.037.544564
GCTAGGC211250.037.1443376
GGCTAGG220900.035.994745
ACGTGGC78200.034.196199
CTGGCTA244550.032.712023
GGTAGTA112050.032.513435
GGCTGGC247150.031.7789521
GCTGGCT256250.030.9257642
Produced by FastQC (version 0.11.5)