FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766996
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT7505264.476210288354575No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG7398944.412800003053618No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3511162.0940900802982236No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG3480462.075780300776597No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT3242841.934061414459692No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC2477361.4775216741269575No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2017381.2031851143758847No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG1730801.0322660063854014No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1723681.0280195689197993No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA1595240.9514166998071689No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1549760.9242919840858791No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC982150.5857638422529593No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA921410.5495379136489327No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC919230.5482377403799703No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG751380.4481303627674272No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT746900.4454584470587337No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG640960.3822747974652108No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA626710.3737759584364426No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG604070.3602732415514383No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA562440.33544470339230714No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA550680.32843092465698687No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT502750.2998450050325055No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA500290.29837783703174975No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT490760.29269405205321214No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC487730.29088693049130565No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG448560.26752556033293023No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT421800.2515656352515382No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC407100.24279841183238787No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG396070.23622001221924308No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG395910.23612458665821834No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG392190.23390594236439252No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA375590.2240055404080731No Hit
GGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCT351760.20979309591294706No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC350030.2087613070343668No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA342970.20455065415414903No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG340200.2028985991289078No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA337860.20150300029892057No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA335540.20011932966406146No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG330080.19686293239409136No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA329830.19671382995499015No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC328440.1958848203935875No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC325160.1939285963925798No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA320830.1913461421473471No Hit
GAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACA315520.18817920634083768No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC313890.18720705843789787No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG309230.1844277889730516No Hit
TACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCT306500.18279959033806653No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG304550.1816365913130772No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG303150.18080161765411049No Hit
AACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCC298940.17829073257964634No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT297560.17746768711580777No Hit
CAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACG296040.17656114428607247No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA286850.1710801386247125No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA283140.16886745842845075No Hit
GACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACAT270940.16159125940031238No Hit
TCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTG266050.158674815691493No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG262600.15661720203189647No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG261300.15584186934857025No Hit
TCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCT255090.15213816476129655No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC251330.14989566407721455No Hit
CATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGG242120.14440273022072647No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC237880.1418739528535702No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC236620.14112247656050017No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC235330.140353107974738No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC232740.1388084067056496No Hit
CTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGA231120.13784222290027384No Hit
GCCCCGGGCGTGGGGGGGGCGGCGGCGCCTCGTCCAGCCGCGGCGCGCGC230150.1372637054365612No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC227600.135742860557729No Hit
CGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTT224320.1337866365567213No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC224140.13367928280056846No Hit
TCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG222970.13298148338557483No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG215400.12846666152959063No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA215110.12829370270023324No Hit
CCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG213320.1272261292362687No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA211840.1263434427967896No Hit
ACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGC210120.12531761801577337No Hit
ACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGG207490.1237490603564288No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC206840.12336139401476567No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT203390.12130378035516917No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA201100.11993800201300221No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG197380.11771935771917642No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT196140.11697980962123448No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA190090.11337153059498553No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG189710.11314489488755172No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT188510.11242920317986596No Hit
TTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGA188340.11232781352127716No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA187620.1118983984966657No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT187010.1115345885452588No Hit
GACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATT183820.10963204142232753No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG183480.1094292621051499No Hit
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC181360.10816487342157177No Hit
GACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTG177580.10591044454236168No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA177560.10589851634723359No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC174390.10400789741943041No Hit
AGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTA173070.1032206365409761No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC172630.10295821624815799No Hit
CCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCACTCCGCACC172590.1029343598579018No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA172350.10279122151636465No Hit
ACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC169550.10112127419843125No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT169390.10102584863740649No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)