FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13863033
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC534780.3857597395894535No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC277000.19981197476771498No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT242970.17526467692892314No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC235260.1697031234074102No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT209940.1514387219593288No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG149790.10804994837709757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA48050.059.609434
GCGACAT49500.057.7652555
CTGCGAC49600.057.657113
GCTGCGA57550.050.112632
CGACATC69350.041.0940176
GGCTGCG71700.039.8956071
CTAGGCG173350.033.454277
TAGGCGG179100.032.3279958
TGGCTAG185750.031.9648174
GCTAGGC183850.031.5188246
GGCTAGG196050.030.0663225
ACCCGAC32200.028.620943
GGCTGGC209800.028.3108061
ACTCGGG180000.028.272531
CTGGCTA224950.027.242173
CTCGGGA187200.027.207762
CCGACTC34400.026.924745
GCTGGCT224000.026.386162
TCGGGAG197550.025.8024373
GACATCT114600.025.2401777
Produced by FastQC (version 0.11.5)