FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13863033
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG237060.17100154057196573No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG167580.12088263802012156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC77250.050.9732938
TCCGACC78200.050.295679
TGTCGGG31850.045.192065
TTTCCGA91300.043.4412577
CGGGGAT157650.041.759568
TCGGGGA161900.040.898057
GTTTCCG88000.039.4573256
CTGTCGG37200.037.296464
GTTCCGT27550.036.046911
CCGTTTC92650.035.9501154
CGTTTCC92900.035.7940865
TCACCCC259050.035.0782859
ATCGGGG113900.034.5719386
GCTCCGT60000.032.8654941
TCTATCG83700.032.8142853
ATAGCGC153950.032.59820694-95
TATCGGG94250.031.6524435
CTATCGG89500.031.3756084
CTCCGTT73150.030.790282
TCCGTTT101800.030.7608433
Produced by FastQC (version 0.11.5)