FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973688
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC264760.2211181717779852No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC193790.1618465421848306No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT159810.13346765006738107No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT123700.10330985741402315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA34750.057.9580084
GCGACAT36400.055.4591985
CTGCGAC39300.051.7352143
GCTGCGA44650.045.4368862
CGACATC50800.039.6448146
GGCTGCG58500.035.4944421
CTAGGCG97300.028.5562977
TAGGCGG98950.028.2243568
ACTCGGG119450.027.208511
GCGCGGT36450.026.5837926
GCTAGGC108150.025.6929286
CTCGGGA127950.025.6590122
TCGGGAG133850.024.7016473
GGCTAGG118800.024.0694485
TGGCTAG126250.022.6499634
CGCGGTG45000.021.637167
TTGATCG47150.021.60767722-23
CCGATAG40700.021.18148890-91
CGGTCGA67250.020.97676368-69
GACATCT98300.020.7765677
Produced by FastQC (version 0.11.5)