FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973688
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG174470.14571116267602763No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG144060.12031380807650909No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA119850.10009447381625444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC60600.059.4180879
TTCCGAC61250.058.7855648
TTTCCGA75850.047.845977
GTTTCCG68100.046.5253836
GCTCCGT49750.042.9767951
CGTTTCC74450.041.411435
CCGTTTC79250.041.189684
CGGGGAT87750.040.8161288
CTCCGTT59150.040.0031932
TCCGTTT80750.039.7784463
GTTCCGT21300.039.259761
TCGGGGA93900.038.244047
TCACCCC212350.036.93329
ATAGCGC113300.036.18060394-95
TGTCGGG22200.035.0938575
CTATCGG49950.034.0548134
CCGATCG122800.033.9032486-87
CGATCGG120850.033.82142386-87
ACCCGAT124750.033.44930384-85
TATCGGG54000.033.4295165
Produced by FastQC (version 0.11.5)