FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14277746
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC530230.37136814172208976No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC355550.2490239005512495No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT190620.1335084683534782No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT185040.12960028844889102No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC145750.10208193926408272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA58450.060.9527554
GCGACAT59100.060.2783665
CTGCGAC64700.055.3634573
CGACATC67400.052.9258356
GCTGCGA71450.050.0749362
GGCTGCG91000.039.737991
TCGACCG14950.039.0858963
CCGCTCG17100.034.4418037
CTAGGCG176050.033.750617
TAGGCGG179100.033.1229258
GCTAGGC193550.030.8481966
GCTCGCG19100.030.8354389
TGGCTAG202850.029.9510564
GGCTAGG205550.029.6018835
CGACCGC21650.027.2069844
GCGCGGT51750.027.0769236
GACATCT133100.026.9776467
ACTCGGG151450.026.826221
CTCGACC22250.026.480082
TTGATCG70000.025.64821222-23
Produced by FastQC (version 0.11.5)