FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8295651
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT834861.0063827419933649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355770.42886326823536813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343220.4137348593859602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156950.18919551943542465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96500.084.502041
GTATCAA299500.070.37251
ATCAACG373050.056.593013
TCAACGC376800.056.029784
CAACGCA381600.055.5589685
AACGCAG397300.053.3604246
ACGCAGA424450.049.875627
CGCAGAG428050.049.511168
TATCAAC429200.049.411722
GCAGAGT461550.045.8917859
GCAGCGC12750.038.732158
TCTGTCG14950.037.410238
GTCTCGC14200.037.3057331
CTCGCTC16700.032.789893
AGAGTAC495750.032.16442510-11
CAGAGTA496400.029.67118510-11
TCTCGCT19050.029.3701882
CGTCAAT5850.028.4789646
AGTACTT509300.028.09019912-13
CGCTCTG17050.027.927645
Produced by FastQC (version 0.11.5)