FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20992395
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC444960.21196247498201137No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC277630.13225265625956448No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT250080.11912885594997616No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT229010.10909188780032007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA53600.054.502754
GCGACAT54100.053.907245
CTGCGAC59400.049.345223
GCTGCGA70350.042.1422352
CGACATC73950.039.501166
GGCTGCG93750.031.8802661
CTAGGCG171700.028.4644287
TAGGCGG178400.027.3159378
ACTCGGG219100.026.3931241
GCTAGGC193000.025.250096
CTCGGGA234450.024.7233122
GGCTAGG216250.023.3921155
TGGCTAG218750.023.0396254
TCGGGAG254850.022.7043483
GCGCGGT58900.022.4182856
TTGATCG69600.021.12140322-23
TCGCACG23750.019.90144342-43
ACGGACC35600.019.747854
TGATCGC75000.019.72746324-25
CTGGCTA262350.019.7193763
Produced by FastQC (version 0.11.5)