FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20142290
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC890130.44192095337719794No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC736040.36542021786003476No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG343330.17045231699076918No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC267660.13288459256618784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA108500.066.3285144
GCGACAT113400.063.5418365
CTGCGAC121450.059.5348433
GCTGCGA129050.056.256442
TCGACCG32900.049.094983
GCTCGCG36050.045.586649
GGCTGCG161800.045.342661
CTCGACC41300.039.2293622
CCGCTCG43600.037.583317
CCTCGAC45150.036.0972861
CGACCGC50550.033.0779764
CTAGGCG314050.032.002167
ACATCTG229800.031.4993558
TAGGCGG321700.031.270998
GCGCGGT114400.030.8495566
GCTAGGC332250.030.350876
TGGCTAG339000.030.183074
CTCGCGT27350.030.13082510-11
CGCGTCG27850.029.84703312-13
GGCTAGG343150.029.677555
Produced by FastQC (version 0.11.5)