FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20142290
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG405730.20143191265739893No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG331810.16473300702154522No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG209010.1037667514468315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC126300.056.3379978
TCCGACC127150.055.851949
TTTCCGA155900.045.9764067
TGTCGGG53000.045.1740045
CGTTTCC135050.044.4969065
GTTTCCG141750.043.964886
CCGTTTC137400.042.6735044
CGGGGAT252900.042.1845938
GTTCCGT33500.041.8420031
GCTCCGT99750.039.9177861
TCGGGGA269450.039.8578837
TCCGTTT142050.039.0373233
CTCCGTT113000.038.601282
CTGTCGG64450.036.566944
GTCGGGG73450.034.6631556
TCACCCC377650.034.4143839
TCTGTCG68300.033.881193
GTCCCGT32950.033.4555241
CCCCGTT56650.033.047132
TCTATCG142950.032.7750323
Produced by FastQC (version 0.11.5)