FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9312459
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC561370.6028160768278281No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC306920.32957997452659926No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC187350.20118209379499014No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG106070.11390117261187406No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA102360.10991726245452463No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG101680.10918705789738242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA48250.063.2084734
GCGACAT48700.062.422285
CTGCGAC50850.059.8854063
GCTGCGA54400.056.154522
GGCTGCG67900.045.551231
CGACATC69350.044.0379876
CTAGGCG172200.038.3103147
TGGCTAG177000.037.9233324
TAGGCGG177150.037.2134258
GCTAGGC182700.036.1619266
GGCTAGG187050.035.729345
GGCTGGC192500.034.380271
CTGGCTA198950.033.716673
GCTGGCT204750.032.4613342
AGGCGGG214050.030.7982169
GCGCGGT36550.030.4082536
GACATCT104100.029.7013997
ACCCGAC25900.029.5311283
ACATCTG109050.028.397058
CGCGGTG40550.027.6418937
Produced by FastQC (version 0.11.5)