FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13457173
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC259730.1930048755410962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA21050.044.0079464
GCGACAT22400.041.139935
CTGCGAC25400.037.2217673
CGACATC27900.033.5375676
CTAGGCG84750.032.9526987
TAGGCGG87100.032.009098
GCTGCGA30750.030.9031562
GCTAGGC108700.026.1301046
GGCTAGG123050.023.5868245
TGGCTAG129800.022.9844194
GGCTGCG43000.022.6528451
GGTGTGC93050.022.4079197
CGGTCGA64800.021.2569368-69
AGGCGGG136200.020.8882989
GGCTGGC138850.020.8063091
TGCGAAC23000.020.23834638-39
GCTGGCT147300.020.1924762
GTGTGCT154350.020.1552458
CCGATAG43200.020.06640890-91
CGCTCGG72900.019.5144664-65
Produced by FastQC (version 0.11.5)