FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111285
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC324310.2473518041900546No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC176260.1344338102634486No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC150920.11510694794598698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT26400.051.6389545
TGCGACA29100.047.178494
CTGCGAC30950.044.054163
GCTGCGA34800.039.727482
CGACATC37100.036.615236
CTAGGCG113350.032.9768837
TAGGCGG116450.031.8950778
GGCTGCG50250.028.5536381
GCTAGGC132900.028.0552626
TGGCTAG144850.026.7943214
GGCTAGG147700.026.0819365
GGCTGGC155800.025.005581
TGCGAAC22600.023.53960238-39
GCTGGCT167000.023.0718122
AGGCGGG163150.023.0565579
GGTGTGC96800.022.3741137
CTGGCTA181300.021.6445223
CCGATAG62150.021.01710190-91
CGGTCGA92600.020.51785368-69
GTGTGCT161900.019.8315328
Produced by FastQC (version 0.11.5)