FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111285
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC28600.042.1889728
TCCGACC28450.042.077469
CCCGTTA15000.036.429693
CGGGGAT94350.035.747678
TCGGGGA97750.034.8930557
CCGTTAT13700.034.6814164
CCCCGTT33600.032.9497642
CCGTTTC46400.032.153564
GTCCCGT17950.031.5004331
TCCGTTT53400.031.4110433
CCCCCGT25850.031.248011
TGTCGGG22200.031.0278175
TTTCCGA39600.030.5897757
GGTCCGT16950.030.5555971
GCTCCGT33300.030.3927041
CTCCGTT40650.030.2743512
GCCGTTA8800.030.2380813
GTCCGTT16350.030.223952
ATCGGGG70950.029.6599886
GTTTCCG36400.029.102026
Produced by FastQC (version 0.11.5)