FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13330457
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC161590.12121864989324822No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG151450.11361200895063088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT33400.045.272915
TGCGACA35050.043.4112934
CTGCGAC42100.036.478433
CGCACTA43600.034.0319027
GCTGCGA48350.031.8602712
GTCCGCA49450.030.0053164
CGACATC54050.028.413896
TCCGCAC52950.028.0219615
TAGCCCA68350.026.5774481
GTGTGCT124450.025.7820768
GGTCGAC114000.025.491516-17
TGTCCGC58650.025.2986033
TAGCCCC49600.024.7023721
TAGGCCC51750.024.6816391
TAGCCCG11000.024.5133551
TAGCCGC11850.024.3518581
GTGTCCG62050.023.8358652
TCGACCC123300.023.5687818-19
GGTGTGC90350.022.5419857
GACCCGT125750.022.48957620-21
Produced by FastQC (version 0.11.5)