FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8383548
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT859011.0246377786588685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365320.43575822551502064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351790.4196194737597972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162030.19327139297109053No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94600.082.613141
GTATCAA300450.071.596441
ATCAACG371800.057.614933
TCAACGC377050.056.8123664
CAACGCA380550.056.2422565
AACGCAG395850.054.1268276
ACGCAGA421850.050.7623027
TATCAAC425850.050.5414052
CGCAGAG424500.050.4442068
GCAGAGT455950.046.964729
TCTGTCG16600.037.2762648
GCAGCGC12950.036.2964748
GTCTCGC16600.035.139331
AGAGTAC490500.032.48468810-11
CTCGCTC17000.031.8606823
CAGAGTA492000.029.7614210-11
TCTCGCT20850.028.5475882
GCTCTAG18150.028.203131
AGTACTT507800.028.17925512-13
CGTCAAT5150.027.7283546
Produced by FastQC (version 0.11.5)