FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14565743
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG253180.1738188021029892No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG252800.17355791599508516No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG210860.14476432819115373No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA161840.11111002027153713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC118550.050.883258
TCCGACC118200.050.795459
TTTCCGA133650.045.0633547
TGTCGGG37400.044.1612635
GTAGCAA236950.041.8039679
GTTTCCG123850.041.501746
TAGTAGC238900.041.0832947
CGTTTCC117850.040.231835
CGGGGAT204850.039.0386548
AGTAGCA254600.038.9786268
TCGGGGA209700.038.3168147
CCGTTTC120050.038.309194
TCACCCC317750.037.1485639
GTTCCGT29450.036.1001131
TCCGTTT124100.035.911633
CTGTCGG46300.034.6479074
GCCCCGT30450.034.6028181
TCTGTCG46250.034.069653
CCGAATT24650.033.6947674
AAACGAG205800.033.66720222-23
Produced by FastQC (version 0.11.5)