FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13504851
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA273080.202208821111762No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG257520.19068703534752068No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG198810.1472137678527516No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG142070.10519923544510042No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG141330.1046512841941018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA301500.046.0684559
AGTAGCA325750.042.4639638
TAGTAGC334100.041.288947
TGTCGGG26900.040.439715
GTACGGT15550.040.33462
CGGGGAT130550.039.6973468
GCCCCGT29300.039.0827641
TCGGGGA134800.038.6219567
CGACCTA9400.038.4168321
TTCGACG5100.035.4037481
GTAGTAG243950.035.302396
GGTACGG17500.035.297271
TTCCGAC38200.035.067068
CGGTGAA71150.035.0517125
AAACGAG271900.034.51110522-23
TCAGTCG258450.034.4129584-85
AGTCGGT257950.034.35075886-87
GCTAGTA37750.034.2276275
ACGAGAA274200.034.1520824-25
GGTAGTA194100.034.091845
Produced by FastQC (version 0.11.5)