FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17771616
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191510.10776172521395916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC37000.032.8075758
TCCGACC41350.028.782879
CCCCGTT55300.028.1356772
TCGCGCG24300.028.099247
CCCGTTT58550.026.9021683
CCGATCG33700.026.7016886-87
CCGTTAT16500.026.442414
CGCGCGT26400.026.2231488
CTCCGTT53950.026.2019422
CGATCGG34200.025.40880486-87
CCGTTTA28400.025.2148114
CCGTTAC14100.024.5527924
CCCGTTA24750.024.1530193
TCCGTTT69000.024.0655063
CTACCGA14100.023.8862441
CCCCCGT54400.023.5436341
CCGTTTC69100.023.5403654
ACCCGAT38500.023.43268484-85
GCCGTTA14600.023.0718043
GCCCCGT48900.022.8934781
Produced by FastQC (version 0.11.5)