FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881734
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG194040.16330949674517203No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG161470.13589767284808765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC62350.054.322798
TCCGACC63450.053.231289
TTTCCGA70900.047.9060757
GTTTCCG67950.044.042216
TGTCGGG26050.042.4892435
CGTTTCC68700.041.764865
GTTCCGT18550.041.4965131
CGGGGAT128350.040.4902468
TCCGTTT71300.040.25343
CCGTTTC70700.039.651234
GCTCCGT47550.039.4718131
CTCCGTT59300.039.3429832
TCGGGGA133400.039.0644387
GTAGCAA135950.037.0386929
TAGTAGC141100.035.4849667
CTGTCGG32900.033.9387634
GCCCCGT28750.033.7157481
CTATCGG70800.032.7499474
CCCCGTT35450.032.5709572
AGTAGCA154500.032.5301558
Produced by FastQC (version 0.11.5)