FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9184825
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1038941.1311483887825844No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1020561.1111371201955398No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG615400.6700182093834124No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG561220.6110296058988604No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC412200.44878372750705653No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG296320.3226191027047331No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT238990.26020092925014904No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC231480.25202439894064393No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC222420.2421603024554088No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC216830.2360741766990661No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC204140.2222579091055083No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG199190.2168685848668864No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA186650.20321563012904437No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA152020.16551213550612015No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG137200.14937682536139774No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA125000.13609404642984488No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC113400.12346451892115529No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC109160.11884820886625494No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC104240.11349154719877624No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC103940.11316492148734461No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA100080.108962337333591No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT99520.1083526360055853No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG97390.10603359345442073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT14400.056.077475
TGCGACA15050.053.655524
AACCCGA211950.050.0590552
ACCCGAC211450.050.0273443
CGACTCC212350.049.748756
CCGACTC213450.049.5594025
CCCGACT214850.049.32494
GACTCCC220100.048.0248877
CTGCGAC17450.047.3640373
ACTCCCT230300.045.774098
GCTGCGA19150.043.6687972
CTCCCTT260300.040.424459
GTATTCG49500.039.5811541
GCTCCCG156500.039.037598
GAACCCG279800.038.6490821
TATTCGT51400.037.9932142
ATTCGTA53750.036.3210373
CCCGGGG169500.036.1896062
CCGGGGC170300.035.7575953
GGCTCCC172850.035.399977
Produced by FastQC (version 0.11.5)