FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11969755
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA13150.035.7504234
GCGACAT13800.035.0978245
CTGCGAC15450.031.0434573
CGACATC15500.030.6314456
CTAGGCG41050.025.2140457
TAGGCGG40950.025.043738
GCTGCGA20550.024.2635692
TGCGCTA16650.020.2437369
GGTGTGC75000.019.6245467
CCGATCG13100.019.38802518-19
CGGTCGA28300.019.05699368-69
CGCTTCG9150.017.910542-43
AGTGCGC19100.017.6491647
TCGGTCG28900.017.59263668-69
GGCTGCG31100.017.5641521
GCTAGGC61200.017.5329976
TAGTGCG18150.017.5265886
CCGATAG26750.017.48987690-91
GTGTGCT125600.017.2375038
CGCTCGG32600.016.83267264-65
Produced by FastQC (version 0.11.5)