FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8148445
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG818221.0041425081718045No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG398550.48911172622506505No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA379190.46535259181353006No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG352790.43295377216143693No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA269850.331167480421111No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG253310.31086912901786784No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG185710.2279085150602354No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG173580.21302223921251234No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA166700.20457891045469412No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA158140.19407383862810634No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG148970.1828201577110725No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA132930.16313542031639164No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA132020.16201864282080813No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA127150.15604204237741065No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG117760.14451837129660935No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG116380.14282479663297723No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT105710.12973027369025625No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC97760.11997381095411455No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC95020.11661120618719277No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA91390.11215636848502997No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA82760.10156539069724346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTA48500.061.3167955
GGTGTTA73150.060.219582
ATACCGG32350.057.832869
GATAGTA83350.057.6444555
GTGCTAG50650.056.6490023
AATACCG33200.056.3061528
CGCGATA3600.055.3303153
TGCTAGT53800.054.1262324
GTGATAG74850.053.9205253
GGTGATA74300.052.8418352
GGTGCTA54900.051.5630152
GTAGCAA731700.050.580159
ATAGTAG107200.050.2641756
AGTAGCA735750.050.209038
TAGTAGC732550.049.989537
GTGTTAG92300.048.5050133
TGATAGT86600.047.8646854
CGACCTA5700.046.5799371
GCCCCGT15850.044.2708781
TTCGACG3000.044.2509351
Produced by FastQC (version 0.11.5)