FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14322627
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1067600.7453939839388403No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG586050.4091777297558611No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG574910.40139982700101035No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA427640.298576511138634No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC425120.29681705737362285No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA408900.28549231925121No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT365140.25493926498260405No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC298500.2084114876411988No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG285510.19934192240012952No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC227840.1590769626270376No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC207130.14461732474077554No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT205430.14343039164533153No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC203830.14231327814373718No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT200700.1401279248562432No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG191090.13341826188729206No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG190970.13333447837467247No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG182360.1273230113442178No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC168160.1174086290175678No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC159760.11154378313419737No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC159170.11113184753048445No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG158800.11087351503324075No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT150060.10477128253078155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTC194050.056.915065
CCCGACT195500.056.544464
ACCCGAC196900.056.217913
CGACTCC196350.056.170136
AACCCGA198350.055.7351152
GACTCCC205850.053.6240047
ACTCCCT218750.050.4617238
TGCGACG14750.047.9602436
GAACCCG261700.043.051741
CTCCCTT257050.042.9204949
GTATTCG104350.041.1031461
TATTCGT105500.040.5555652
ATTCGTA111550.038.3560033
TCGACGC13550.038.1921737
GTCGACG7650.037.8578156
GTGCGAC15400.037.615795
TTCGTAC114400.037.3983654
CTAGGCG47150.034.4374967
TCGATCG169950.032.69613616-17
TAGGCGG50350.032.0602268
Produced by FastQC (version 0.11.5)