FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16172393
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC354560.21923780852963443No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA308430.1907138912590116No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC296110.1830959710167815No Hit
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA293230.1813151584926238No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT179480.11097924716521544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT58800.061.1525735
TGCGACA59700.060.5558624
CTGCGAC63000.057.3890383
CGACATC64850.055.443236
GCTGCGA68800.053.312762
GGCTGCG86850.042.288671
CGGTCGA47200.030.64258668-69
TCGGTCG49900.029.46050568-69
CTAGGCG105800.028.775687
TAGGCGG106000.028.7213848
TTGATCG64200.028.11404222-23
TGATCGC66250.027.56689524-25
CGCTCGG56300.027.12401864-65
ATTCGGC67600.027.12222938-39
TTCGGCT68150.026.87065340-41
GCGCGCT58750.026.842362-63
CGACCAT55000.026.51535482-83
GATCGCC69400.026.48676324-25
TGATTCG69200.026.1180536-37
GGTCGAA55100.025.99101370-71
Produced by FastQC (version 0.11.5)